Using cryo-EM, the He Laboratory revealed how TFIIIA, TFIIIC, and TFIIIB assemble on the S. cerevisiae 5S rRNA promoter, with TFIIIA bridging TFIIIC-DNA interactions and TFIIIB inducing gene wrapping. smFRET showed DNA bending and partial dissociation, offering insights into Pol III transcription initiation and its distinction from Pol II.
Structural basis of TFIIIC-dependent RNA polymerase III transcription initiation
Yuan He
and Anna Talyzina, Yan Han, Chiranjib Banerjee, Susan Fishbain, Alexis Reyes, Reza Vafabakhsh |
Molecular Cell, 2023