Structural basis of TFIIIC-dependent RNA polymerase III transcription initiation

Yuan He and Anna Talyzina, Yan Han, Chiranjib Banerjee, Susan Fishbain, Alexis Reyes, Reza Vafabakhsh | Molecular Cell, 2023 

Using cryo-EM, the He Laboratory revealed how TFIIIA, TFIIIC, and TFIIIB assemble on the S. cerevisiae 5S rRNA promoter, with TFIIIA bridging TFIIIC-DNA interactions and TFIIIB inducing gene wrapping. smFRET showed DNA bending and partial dissociation, offering insights into Pol III transcription initiation and its distinction from Pol II.