How chromatin-based enzymes find nucleosome-free genomic targets in the cell nucleus has been enigmatic. Using cryo-EM, biochemistry and epigenomics, we found a generalizable mechanism showing that the ATP-driven yeast SWR1 remodeler senses accessible chromatin architecture with a protein module that measures free DNA linked flexibly to histone PTM reader domains, enabling SWR1 capture and histone H2A.Z exchange on flanking acetylated nucleosomes at gene promoters.
Molecular basis of global promoter sensing and nucleosome capture by the SWR1 chromatin remodeler
Carl Wu
and Robert K. Louder, Giho Park, Ziyang Ye, Justin S. Cha, Anne M. Gardner, Qin Lei, Anand Ranjan, Eva Hollmuller, Florian Stengel, B. Franklin Pugh |
Cell, 2024